Transfection Cell Database - Detailed View


Cell Line: Primary astrocytes

Cell Line Species/Tissue: rat / brain 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10 
Reporter System:
CAT 
Plasmid Purification Method:
QIAfilter Plasmid Kit 
Plate Format:
6-well plate 
Number of Cells:
 
Percent Confluence(%):
80 
Amount of Nucleic Acid (µg):
0.4 
Amount of Enhancer (µl):
3.2 
Amount of Reagent (µl):
10 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
control vector/basic vector = ca. 500/1 
 
Any modifications to the protocol?:
 
Notes:
 
References: