DNeasy mericon Food Kit
For extraction of high-quality DNA from raw or processed foods
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DNeasy mericon Food Kit (50)
Cat. No. / ID: 69514
50 QIAquick Spin Columns, Proteinase K, buffers
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The DNeasy mericon Food Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Modified CTAB procedure ensures faster food sample processing
- Efficient purification of even fragmented DNA from processed foods
- No carryover of PCR inhibitors from complex food matrices
- Protocols for highly processed foods
Product Details
The DNeasy mericon Food Kit is designed to enable extraction of high-quality nucleic acids from a variety of raw and processed foods. The carryover of PCR inhibitors from these complex food matrices is minimized. The kit forms part of the comprehensive QIAGEN food testing portfolio, which also features assays for pathogen and GMO DNA detection and ingredient authentication. The procedure can be fully automated on the QIAcube Connect.
Performance
During production, food is subjected to heat, irradiation, high pressure, and changes in pH which lead to DNA degradation and fragmentation. The optimized chemistry of the DNeasy mericon Food Kit was developed to recover short DNA fragments (down to 100 bp) ensuring that even highly fragmented DNA is efficiently isolated and subsequently amplified in PCR reactions. With these features, extraction with the DNeasy mericon Food Kit is the first universally applicable extraction method that generates optimal and reliable results even when using strongly inhibitory, highly processed, fatty, acidic, high, or low DNA content foods (see Figure "High yields of DNA and minimal inhibitor carryover" and Figure "Heightened resistance to inhibitors"). In addition, establishing multiple lysis procedures for different food matrices is no longer necessary.
Principle
The DNeasy mericon Food Kit uses improved cetyltrimethylammonium bromide (CTAB) extraction of total cellular nucleic acids.
The nonionic detergent CTAB is widely used for efficient extraction of total cellular nucleic acids from a wide range of tissue types. Depending on the salt conditions, CTAB may complex with cellular nucleic acids (low-salt conditions) or complex with cellular inhibitors, such as polysaccharides, proteins, and plant metabolites (high-salt conditions; as found in the Food Lysis Buffer).
With fewer workflow steps than conventional CTAB protocols, up to 30 samples can be processed in 2.5 hours (see Figure "Efficient and rapid DNA extraction"). DNA recovery with these kits is demonstrably high and efficient.
Procedure
The optimized protocols for the DNeasy mericon Food Kit use CTAB in combination with Proteinase K to first digest compact tissue and then to subsequently precipitate proteins with simultaneous precipitation of other cellular and food-derived inhibitors.
Inhibitors are precipitated by centrifugation, while the extracted DNA remains in solution. In the subsequent chloroform extraction, any remaining CTAB-protein, CTAB-debris, or CTAB-polysaccharide complex not precipitated is removed together with other lipophilic inhibitors by extraction into the organic chloroform phase. Only the aqueous phase containing the DNA and significantly depleted inhibitors is processed further. This phase is mixed with binding buffer (to adjust binding conditions) and applied to the QIAquick Spin Columns. The resulting DNA is immediately ready for use in a downstream mericon real-time PCR assay.
Applications
The DNeasy mericon Food Kit is designed for rapid (up to 30 extractions in 2.5 hours) purification of DNA from a variety of raw and processed food matrices, while minimizing the carryover of PCR inhibitors inherent to complex food samples.
Supporting data and figures
High yields of DNA and minimal inhibitor carryover.
Samples of peanut butter and chocolate were prepared using the DNeasy mericon Food Kit (standard protocol) and products from 3 other suppliers to amplify a segment of the tnrL chloroplast tRNA gene. (A) Average quantification value (Cq) is substantially lower for samples prepared with the DNeasy mericon Food Kit compared to the other suppliers. (B) Using inhibitor-free DNA, this assay gives a positive Cq shift between 2.9–3.5 when samples are diluted 1:10; lower shifts indicate the presence of inhibitors. To test for inhibitors carried over from the sample preparation procedure, samples were diluted 1:10 and the same DNA segment was amplified. Both food samples were inhibitor-free when prepared with the DNeasy mericon Food Kit. Samples prepared with products from the other suppliers showed strong inhibitor contamination.
Resources
Kit Handbooks (1)
Safety Data Sheets (1)
Brochures & Guides (3)
Certificates of Analysis (1)
FAQ
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
Does salt concentration in the food matter when using the DNeasy mericon Food Kit?
CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use?
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?