Transfection Cell Database - Detailed View


Cell Line: NCI-H460

Cell Line Species/Tissue: Human / Lung 
Transfection Reagent: Attractene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM F12 glutamax + 7.5% FCS 
Percent Serum (%):
7.5 
Reporter System:
B-galactosidase 
Plasmid Purification Method:
Macherey-Nagel Nucleobond PC500 
Plate Format:
24-well plate 
Number of Cells:
20000 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0.4 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
0.75 
Complex Incubation on Cells (hrs):
16 
Analysis Performed Post-Transfection (hrs):
40 
Transfection Efficiency (%):
30 units 
 
Any modifications to the protocol?:
 
Notes:
Tried 0.2, 0.4 and 0.6ug DNA with 1.5ul Attractene reagent, and 0.4ug DNA with 0.75, 1.5 and 2.25ul reagent. 0.4ug+0.75ul gave the best results. Trend in the data: the more DNA the better, and the less reagent the better.  
References: