| Cell Line Species/Tissue: |
human
/
|
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI 1640 |
Percent Serum (%):
|
10% FCS |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
Endofree Plasmid Kit |
Plate Format:
|
24-well plate |
Number of Cells:
|
20000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
2 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
22 µl |
Complex Incubation on Cells (hrs):
|
7 hrs |
Analysis Performed Post-Transfection (hrs):
|
48-72 hrs |
Transfection Efficiency (%):
|
10 % |
| |
| Any modifications to the protocol?:
|
| no |
| Notes: |
| |
| References: |
| |