| Cell Line Species/Tissue: |
Human
/
kidney epithelial |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
Dmem/FBS/Pen/strep |
Percent Serum (%):
|
10 % |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
40-80 % |
Amount of Nucleic Acid (µg):
|
0.4 µg |
Amount of Enhancer (µl):
|
3.2 µl |
Amount of Reagent (µl):
|
10 µl |
Complex Incubation on Cells (hrs):
|
18 h |
Analysis Performed Post-Transfection (hrs):
|
24-48 h |
Transfection Efficiency (%):
|
10-30% |
| |
| Any modifications to the protocol?:
|
| Different cell densities, removing after 8, 16, 24 hrs of the transfection complex |
| Notes: |
| |
| References: |
| |