| Cell Line Species/Tissue: |
Human
/
Embryonic Kidney |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM/Pen/Strep |
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
Invitrogen Maxi Kit |
Plate Format:
|
24-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
70% |
Amount of Nucleic Acid (µg):
|
0.3µg |
Amount of Enhancer (µl):
|
1.6µl |
Amount of Reagent (µl):
|
5µl |
Complex Incubation on Cells (hrs):
|
6hrs |
Analysis Performed Post-Transfection (hrs):
|
48hrs |
Transfection Efficiency (%):
|
50% |
| |
| Any modifications to the protocol?:
|
| No serum during complex incubation. Used 0.3ug DNA instead of 0.2ug. |
| Notes: |
| o serum during complex incubation. Used 0.3ug DNA instead of 0.2ug. |
| References: |
| |