| Cell Line Species/Tissue: |
Human
/
Glioma |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM, penicillin (100 units/ml) and streptomycin (100 µg/ml) |
Percent Serum (%):
|
10% heat-inactivated FBS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
no information |
Number of Cells:
|
|
Percent Confluence(%):
|
no information |
Amount of Nucleic Acid (µg):
|
no information |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
no information |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
24 hrs |
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| PTEN Suppresses Hyaluronic Acid-induced Matrix Metalloproteinase-9 Expression
in U87MG Glioblastoma Cells through Focal Adhesion Kinase Dephosphorylation1
Myung-Jin Park, Mi-Suk Kim, In-Chul Park, Hee-Seok Kang, Heon Yoo, Seok Hee Park, Chang Hun Rhee,
Seok-Il Hong, and Seung-Hoon Lee
CANCER RESEARCH 62, 6318–6322, November 1, 2002 |