Transfection Cell Database - Detailed View


Cell Line: P-19

Cell Line Species/Tissue: Mouse / Embryonal Carcinoma 
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
DMEM/ High Glucose 
Percent Serum (%):
10% 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
96-Well 
Number of Cells:
5,000 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
75 nM 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
2 µl 
Complex Incubation on Cells (hrs):
48 hrs 
Analysis Performed Post-Transfection (hrs):
48 hrs 
Transfection Efficiency (%):
 
Knockdown Efficiency (%): 78%  
 
Any modifications to the protocol?:
 
Notes:
Modification of Rervese Transfection Protocol: 1. Spotting of siRNA in 15 µl (medium without serum), 2. Addition of HiPerFect-dilution (15 µl per well; medium without serum), 3. incubation for 10 minutes at RT, 4. Addition of 30 µl cell suspension in medium + serum, 5. After 6 hours addition of 140 µl medium + serum.  
References: