Transfection Cell Database - Detailed View


Cell Line: HeLa

Cell Line Species/Tissue: Human / Cervix; epithelial; adenocarcinoma 
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
DMEM/F12 
Percent Serum (%):
10 % 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
60 mm 
Number of Cells:
6x10e5 
Percent Confluence(%):
90 % 
Amount of Nucleic Acid (µg):
1nM,10nM,5nM 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
5 µl 
Complex Incubation on Cells (hrs):
24 h, 48 h 
Analysis Performed Post-Transfection (hrs):
24 h, 48 h 
Transfection Efficiency (%):
60~70% 
Knockdown Efficiency (%): 83.40 %  
 
Any modifications to the protocol?:
Yes, see notes  
Notes:
Amount of HiPerFect Reagent :20µl?5µl (in 60mm Dish) Because 20µl of transfection reagent made quite many of cells died, but when I used 5µl transfection reagent, almost cells was not dead and transfection has been successful. Analysis method: real-time PCR  
References: