Transfection Cell Database - Detailed View


Cell Line: SK-N-SH

Cell Line Species/Tissue: Human / Neuroblastoma 
Transfection Reagent: RNAiFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
DMEM 
Percent Serum (%):
 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
2cm *2 dishes 
Number of Cells:
no information 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0.8 µg -1.2 µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
6 µl 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
no information 
Transfection Efficiency (%):
no information 
Knockdown Efficiency (%): no information  
 
Any modifications to the protocol?:
 
Notes:
siRNA from QIAGEN: c-Rel siRNA (0.8 µg) or Bcl-XL siRNA (1.2 µg) To transfect cortical cultures, the transfection complex was diluted in 300 µl of Neurobasal/B27 and added directly to the cells. It was replaced with fresh Neurobasal/B27 3 h later. To transfect SK-N-SH cells, the transfection complex was added in DMEM without serum and antibiotics. It was replaced with complete DMEM 3 h later.  
References:
Cell Death and Differentiation (2005), 1–12 NF-jB factor c-Rel mediates neuroprotection elicited by mGlu5 receptor agonists against amyloid b-peptide toxicity M Pizzi*,1, I Sarnico1, F Boroni1, M Benarese1, N Steimberg2, G Mazzoleni2, GPH Dietz3, M Ba hr3, H-C Liou4 and PF Spano