| Cell Line Species/Tissue: |
Human
/
Cervix; epithelial; adenocarcinoma |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
|
Percent Serum (%):
|
10 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well plate |
Number of Cells:
|
2,3x10*5 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.3 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
12 µl |
Complex Incubation on Cells (hrs):
|
72-96 h |
Analysis Performed Post-Transfection (hrs):
|
72 h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
85 % |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Target:pom |
| References: |
| |