| Cell Line Species/Tissue: |
Human / T-cell leukemia
/
|
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
10% |
Reporter System:
|
expression vector & synthetic siRNA |
Plasmid Purification Method:
|
QIAGEN EndoFree Plasmid Kit |
Plate Format:
|
24-well |
Number of Cells:
|
200.000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0,4µg |
Amount of Enhancer (µl):
|
1,6µl |
Amount of Reagent (µl):
|
4µl |
Complex Incubation on Cells (hrs):
|
48 h |
Analysis Performed Post-Transfection (hrs):
|
48 h |
Transfection Efficiency (%):
|
30% |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| no complex removal; cells plated in 100 µl RPMI (no serum), complex added in a volume of 100 µl; addition of 200 µl RPMI/ 20% FCS after 3h |
| Notes: |
| Co-transfection of DNA and siRNA |
| References: |
| |