| Cell Line Species/Tissue: |
human
/
T-cell lymphoma |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
10 |
Reporter System:
|
pCMVß ( Clontech ) |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
12-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
6 |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
11 ß-gal units/mg total protein |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| Hill, S., Weiler, S., and Heidecker, G. (1998) Transfection of hematopoetic cells in suspension using an activated-dendrimer reagent. QIAGEN News 1998 No. 1, 1 |