| Cell Line Species/Tissue: |
Mouse
/
Embryo fibroblast |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 |
Reporter System:
|
GFP, pTracer-SV40 |
Plasmid Purification Method:
|
EndoFree Plasmid Kit |
Plate Format:
|
96-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
60-80% |
Amount of Nucleic Acid (µg):
|
0.1 |
Amount of Enhancer (µl):
|
0.8 |
Amount of Reagent (µl):
|
4 |
Complex Incubation on Cells (hrs):
|
6 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
30-40% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Incubated transfection complexes for up to 20h without increase in toxicity. |
| References: |
| |