GelPilot markers are provided ready to load in gel loading buffer. The unique buffer contains 3 tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time (see figure "GelPilot Loading Buffer"). No additional reagents or dilution steps are required – simply load and run.
To help quantify sample DNA, each GelPilot Molecular Weight Marker consists of standard and intensive DNA fragments. Standard DNA fragments consist of 50 ng DNA, while intensive fragments contain 100 ng DNA (when using 6 µl of marker).
Simply mix the ladder gently by pipetting up and down a few times and load 6 µl into the required number of gel lanes.