- Crude lysate에서 95% pure protein을 얻는데 까지 one-step
- High binding affinity and high capacity
- Native 또는 denaturing conditions Purification선택 가능
- 다양한 정제 scale별 precharged, ready-to-use matrices
- 자동화 분리 정제와 분석 프로토콜
Ni-NTA Agarose is an affinity chromatography matrix for purifying recombinant proteins carrying a His tag. Histidine residues in the His tag bind to the vacant positions in the coordination sphere of the immobilized nickel ions with high specificity and affinity. Cleared cell lysates are loaded onto the matrices. His-tagged proteins are bound, and other proteins pass through the matrix. After washing, His-tagged proteins are eluted in buffer under native or denaturing conditions.