BioRobot EZ1 시스템과 BioRobot EZ1 Advanced 시스템을 이용하여 viral nucleic acids를 자동으로 분리 정제할 수 있습니다
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
EZ1&2 Virus Mini Kit v2.0 (48)
Cat. No. / ID: 955134
For 48 preps: Reagent Cartridges (Virus Mini v2.0), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer AVE, carrier RNA
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EZ1 Virus Mini Kit v2.0은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
특징
- Simultaneous purification of viral DNA and RNA
- Higher analytical sensitivity, even with low viral titers
- Highly reproducible results
- Linear yields from a wide range of viral titers
- Prefilled, sealed reagent cartridges for easy handling
제품 세부 정보
The EZ1 Virus Mini Kit v2.0 provides a fully automated procedure for simultaneous purification of viral DNA and RNA from serum, plasma, CSF, urine, respiratory samples, and other cell-free body fluids for sensitive analytical detection in downstream assays. The kit provides optimized binding conditions for more robust and reproducible capture of nucleic acids, and improved wash conditions for higher analytical sensitivity in downstream assays. The kit can be used with the EZ1 Advanced XL (1–14 samples per run), the EZ1 Advanced (1–6 samples per run), and the BioRobot EZ1 (1–6 samples per run). The EZ1 DSP Virus Kit is also available for viral nucleic acid purification for in vitro diagnostics (not available in all countries).
원리
Automated purification with the EZ1 Virus Mini Kit v2.0 requires use of the EZ1 Advanced XL and the EZ1 Advanced XL Virus Card v2.0, or the EZ1 Advanced and the EZ1 Advanced Virus Card v2.0, or the BioRobot EZ1 and the EZ1 Virus Card v2.0. EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.
Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1-14 samples (EZ1 Advanced XL) or 1-6 samples (EZ1 Advanced and BioRobot EZ1) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases.
절차
Serum or plasma samples (100 µl, 200 µl, or 400 µl) are loaded onto the instrument, and viral nucleic acids are purified in one step following the automated EZ1 Virus procedure. Viral nucleic acids are eluted in a volume of 150 µl, 120 µl, 90 µl, or 60 µl and are ready to use in downstream applications.
The instrument makes viral nucleic acid purification convenient and reliable. The EZ1 Virus Mini Kit v2.0 provides prefilled, sealed reagent cartridges that help to prevent human error during setup of the instrument, while the EZ1 Cards set the purification protocol without any manual data entry. Fully automated purification from 1–6 samples requires only 40 minutes, including only 5 minutes hands-on time.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time RT-PCR |
| Elution volume | 50–200 µl |
| Technology | Magnetic particles |
| For automated processing | EZ2 Connect instruments or EZ1 Advanced XL |
| Sample amount | 100 µl, 200 µl, 400 µl |
| Processing | Automated |
| Format | Sample tube |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral DNA, viral RNA |
| Time per run or per prep | 40 minutes |
| Main sample type | Serum, plasma |
리소스
Quick-Start Protocols (3)
Kit Handbooks (2)
Brochures & Guides (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
Is training of lab personnel included with the purchase of a BioRobot EZ1?
How can we get our BioRobot EZ1 upgraded when new protocols are launched?
Where can I find information about the worktable setup on the BioRobot EZ1?
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
What disposables are required for nucleic acid isolations on the BioRobot EZ1?