QIAsymphony DSP HPV Media Kit

For automated sample preparation of human cervical cells stored in liquid-based cytology media

Features

Prefilled reagent cartridges, sufficient for 2 x 352 sample preparations
High reproducibility through standardized processing
Processes up to 88 samples, in 24 batches per run, in less than 2.5 hours
Bar codes for complete tracking of samples and reagents
For in vitro diagnostic use

Product Details

The QIAsymphony DSP HPV Media Kit, in combination with the QIAsymphony SP, provides sample extracts that are ready for automated testing using the digene HC2 High-Risk HPV DNA Test with the Rapid Capture System. This kit uses QIAsymphony magnetic particle technology for automated isolation of human cervical cells stored in liquid-based cytology (LBC) media.

Principle

QIAsymphony DSP HPV Media technology combines the speed and efficiency of pH-driven anion exchange chromatography with the convenient handling of magnetic particles. Ready-to-run reagent cartridges are prefilled with all the reagents required for the sample preparation procedure and are automatically opened by the QIAsymphony SP (), which helps to minimize the risk of environmental contamination.

See figures

Schematic of the QIAsymphony SP principle.

Procedure

The sample preparation procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises binding and recovery of sample extracts. Worktable setup is rapid, which saves valuable time. Simply check the waste drawer, load consumables, and load 2 reagent cartridges. Reagent volumes are used only for the selected number of samples, allowing complete cost control.

Protocol for use with the QIAsymphony DSP HPV Media Kit
Protocol	Sample type	Volume processed
PC2500_HC2_V1_DSP	PreservCyt samples	2.5 mL
SP800_HC2_V1_DSP	SurePath specimens/post-gradient cell pellet samples	800 µL

Applications

The QIAsymphony DSP HPV Media Kit, when automated on the QIAsymphony SP, enables the sample preparation of specimens that have been collected in liquid-based cytology media, such as PreservCyt Solution (Hologic) or SurePath Preservative Fluid.