AllStars Hs Cell Death Control siRNA

siRNA transfection 실험의 최적화 및 siRNA control실험으로 사용할 수 있습니다

Products

AllStars Hs Cell Death Control siRNA은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.

AllStars Hs Cell Death siRNA (1 nmol)

Cat. No. / ID: SI04381048

Positive cell death phenotype control, 1 nmol

GeneGlobe에서 구성

AllStars Hs Cell Death siRNA (5 nmol)

Cat. No. / ID: 1027298

Positive cell death phenotype control, 5 nmol

Inquire

AllStars Hs Cell Death siRNA (20 nmol)

Cat. No. / ID: 1027299

Positive cell death phenotype control, 20 nmol

Inquire

특징

Optimization하는데에 사용하기 쉽고 positive control로 사용 가능
High-throughput RNAi를 위한 valuable control
Light microscopy을 통한 간편한 evaluation
다양한 종류의 세포형태에 효율적

제품 세부 정보

AllStars Hs Cell Death Control siRNA is a blend of highly potent siRNAs targeting ubiquitously expressed human genes that are essential for cell survival. Knockdown of these genes induces a high degree of cell death, which is visible by light microscopy. Transfection efficiency can be quickly estimated by simply observing cells by straightforward light microscopy 48–96 hours after transfection of AllStars Hs Cell Death Control siRNA, avoiding the need for any complex or laborious downstream assay. AllStars Hs Cell Death Control siRNA is a useful tool for siRNA transfection optimization and can be used routinely as a positive control.

성능

Fast and easy detection in a wide range of cell types

The performance of AllStars Hs Cell Death Control siRNA is validated for a wide range of cell types (see table).

AllStars Hs Cell Death Control siRNA Validated Cell Types
Cell line	Cell type
293	Human embryonic kidney cells
A549	Human alveolar basal epithelial cells
CaCo	Human colonic carcinoma cells
HeLa	Human cervical cancer cells
HeLa S3	Human cervical cancer cells
HepG2	Human hepatoma cells
Huh7	Human hepatoma cells
MCF-7	Human breast adenocarcinoma cells
MDA-MB-231	Human breast adenocarcinoma cells
ME180	Human cervical squamous carcinoma cells
SW 480	Human colon adenocarcinoma cells
Primary cells	Cell type
HUVEC	Primary human umbilical vein endothelial cells
NHBE	Primary normal human bronchial epithelial cells
NHLF	Primary normal human lung fibroblast cells

Highly efficient knockdown and cell death

Knockdown efficiency using AllStars Hs Cell Death Control siRNA has been quantified by real-time RT-PCR and is >95% for each target mRNA.

The AllStars Hs Cell Death Control significantly outperforms other commercially available toxic control siRNAs in both the level of cell death induced and in the wide range of cell types in which it has been validated. Consistently high levels of cell death are achieved using AllStars Hs Cell Death Control, ensuring effective control experiments (see figure " High level of cell death").

그림 참조

High level of cell death.

원리

Easy transfection optimization

When establishing RNAi in start-up experiments or in a new cell line, multiple transfections should be performed under different conditions to determine the optimal conditions for maximum transfection efficiency. These experiments can be performed using AllStars Hs Cell Death Control siRNA (see figures Easy siRNA transfection optimization and Cell death in primary cells). Transfection conditions that result in the greatest degree of cell death compared with transfection with a nonsilencing control siRNA can be maintained in future experiments.

Reliable high-throughput RNAi

In high-throughput RNAi screens, the use of internal positive controls is essential to ensure that optimal transfection conditions are maintained on every plate of the screen. Positive controls that can be analyzed phenotypically have the advantage of allowing transfection efficiency to be observed immediately. Using AllStars Hs Cell Death Control siRNA on every plate allows rapid and inexpensive estimation of the transfection rate on individual plates.

그림 참조

Easy siRNA transfection optimization.

Cell death in primary cells.

절차

Using AllStars Hs Cell Death Control siRNA, transfection efficiency can be quickly estimated by simply observing cells by straightforward light microscopy 48–96 hours after transfection, avoiding the need for any complex or laborious downstream assay.

AllStars Hs Cell Death Control siRNA can be ordered in tube format in 1 nmol, 5 nmol, or 20 nmol amounts. This control is also available in 96-well plates and 384-well plates (FlexiPlate siRNA) in 1 nmol, 0.25 nmol, or 0.1 nmol amounts.

응용 분야

Start-up RNAi experiments
Transfection optimization
High-throughput RNAi

지원되는 데이터 및 수치

High level of cell death.

[A] SW620 cells or [B] SW480 cells (5 x 10³cells per well in 96-well plates) were transfected with 20 nM of AllStars Hs Cell Death Control siRNA or AllStars Negative Control siRNA or an siRNA targeting PLK (a well-known target for induction of cell death). Untransfected cells and cells treated with transfection reagent only were also analyzed. After 72 hours, cell death was quantified using a CellTiter-Blueassay (Promega). AllStars Hs Cell Death Control siRNA resulted in high levels of cell death in both cell types. (Data kindly provided by Dr. Amanda Hummon and Dr. Thomas Reid, National Cancer Institute, Maryland, U.S.A.)

Specifications

Features	Specifications
Design	Designed using HP OnGuard siRNA design
Format	Tube
Scale or yield	5 nmol, 20 nmol
Target sequence provided	No
Modification	Yes
Species	Human

리소스

FAQ

You can add the following controls to your FlexiPlate siRNA plate: AllStars Negative Control siRNA, AllStars Cell Death Control siRNA, Negative Control siRNA, Human GAPDH siRNA, Human Beta-Actin siRNA, Human and mouse MAPK1 siRNA, Human or mouse Lamin A/C siRNA, Mouse AKT1 siRNA, or other siRNAs from GeneGlobe, such as HP Validated siRNAs.

FAQ ID -1368

No, we have qRT-PCR and phenotypic data (using AllStars Cell Death Control siRNA). We do not have data with fluorescently labeled siRNA.

FAQ ID -2557