✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
AllPrep RNA/Protein Kit (50)
Cat. No. / ID: 80404
50 AllPrep Mini Spin Columns, 50 RNeasy Mini Spin Columns, 50 Protein Cleanup Mini Spin Columns, Collection Tubes, RNase-Free Reagents and Buffers
로그인 계정 가격 보기.
AllPrep RNA/Protein Kit은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
특징
- 같은 샘플로 부터 high-quality RNA와 protein을 편리하게 분리 정제할 수 있음
- 빠른 실험 과정 — 간소화된 spin-column 방식의 protocol
- 적은량의 cell cultures 사용 — RNA, protein 정제 블필요
- Ready-to-use RNA and protein — high performance in downstream applications
제품 세부 정보
The AllPrep RNA/Protein Kit allows the simultaneous purification of total RNA and native protein from the same cultured cell sample.
성능
The AllPrep RNA/Protein Kit is designed to purify total RNA and native protein simultaneously from the same sample of cultured cells. Analysis of purified RNA from a sample of cells on the Agilent 2100 Bioanalyzer typically gives RIN values of 10, indicating intact RNA. The native protein purified from the same cell sample contains all cellular proteins (see figure “ Highly intact RNA and high-quality protein from the same cell sample”). RNA purified with the AllPrep RNA/Protein Kit is suitable for real-time RT-PCR (see figure " Sensitivity in real-time RT-PCR") and purified cellular proteins demonstrate functionality in downstream enzyme assays (see figure " Purification of functional protein").
그림 참조
원리
The AllPrep RNA/Protein Kit is designed to purify total RNA and native protein simultaneously from the same sample of cultured cells. For experiments to validate siRNA-mediated knockdown at the mRNA and protein levels, the kit streamlines sample preparation and allows the number of cell cultures to be significantly reduced. In addition, knockdown analysis is more reliable since RNA and protein come from the same sample.The AllPrep RNA/Protein Kit is part of QIAGEN’s range of closely integrated products for RNAi and gene expression analysis. These products provide highly reproducible and sensitive results. Products include optimized PCR master mixes, ready-to-run assays, siRNAs, and transfection reagents. Standardization of RNAi and gene expression analysis workflows by these products have contributed to success in many areas of biomedical research.
절차
The spin-column–based procedure enables purification of RNA and protein from a single cell sample in less than 40 minutes (see flowchart " AllPrep RNA/Protein procedure"). A method is available for simultaneous purification of DNA, RNA, and protein (suitable for western blotting, not for enzyme assays) from cultured cells and tissues using the AllPrep DNA/RNA/Protein Kit.
그림 참조
응용 분야
The AllPrep RNA/Protein Kit allows purification of RNA and protein from a single cell culture. The purified RNA and protein provide high performance in downstream applications, such as real-time RT-PCR, western blotting, and enzyme assays. (Note: The functionality of any protein of interest in an assay has to be tested according to its properties.)
지원되는 데이터 및 수치
Highly intact RNA and high-quality protein from the same cell sample.
Total RNA was purified from HeLa cells and analyzed on the Agilent 2100 Bioanalyzer. The rRNA ratio (28S/18S) was 2.04. Total protein was purified from HeLa cells and analyzed by western blot. Analysis of duplicate protein samples with antibodies against Lamin (numclear protein) and TIM23 (mitochondrial protein) demonstrates that the total protein fraction contains proteins from subcellular compartments.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time PCR, blotting, microarray |
| Elution volume | 30–50 µl |
| Format | Spin column |
| Processing | Manual (centrifugation) |
| Technology | Silica technology |
| Time per run or per prep | 40 minutes |
| Sample amount | 700 µl |
| Main sample type | Cultured cells |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA, native protein |
리소스
Kit Handbooks (2)
Brochures & Guides (1)
Technical Information (1)
Safety Data Sheets (1)
Quick-Start Protocols (1)
Certificates of Analysis (1)
FAQ
What is the composition of Buffer APL?
Can the AllPrep RNA/Protein Kit be used with tissue?
What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ?