Bone marrow 샘플을 안정화하여 보관과 이동을 할 수 있고 intracellular RNA를 분리 정제할 수 있습니다
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특징
- 통합된 system — storage, stabilization, and intracellular RNA purification from bone marrow
- 즉각적인 intracellular RNA stabilization으로 in vivo snapshots에 적합
- Heterogeneous bone marrow에서의 high-quality RNA의 신속한 분리 정제— cell rich sample 포함
- 안전하고 쉬운 운반 — 드라이아이스, 액체질소등 불필요
제품 세부 정보
The PAXgene Bone Marrow RNA System consists of PAXgene Bone Marrow RNA Tubes, for stabilization, storage, and transportation of bone marrow, and the PAXgene Bone Marrow RNA Kit, for silica-membrane-based RNA isolation and purification in a spin-column format. Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is immediately transferred into a PAXgene Bone Marrow RNA Tube, which stabilizes the RNA in the sample for storage at room temperature, refrigerated, or frozen. RNA can be purified days later using the PAXgene Bone Marrow RNA Kit or archived for several months at –20°C or –70°C.
성능
The PAXgene Bone Marrow RNA Kit enables efficient isolation and purification of intracellular RNA from heterogeneous human whole bone marrow samples (see figure " RNA yields from bone marrow samples"), including an optimized procedure for cell-rich, viscous samples. RNA can be purified days after collection, and intact, high-quality RNA can be purified using the PAXgene Bone Marrow RNA Kit after samples are stored at room temperature, refrigerated, or frozen (see figures " RNA stability at room temperature", "RNA stabilized at 2–8°C", and "High RNA integrity after freeze–thaw cycles"). RNA Tubes protect RNA molecules from degradation by RNases and enable accurate analysis by minimizing ex vivo changes in gene expression (see figure " Stability of 5 transcripts at room temperature"). An integrated DNase treatment ensures that genomic DNA is minimized. In-house and external studies showed that genomic DNA was <0.85% in 95% of the samples tested (n = 157). A260/A280 values are typically between 1.8 and 2.2, indicating the high purity of the RNA (see figure " Storage under different conditions").
그림 참조
원리
Copy numbers of individual mRNA species in bone marrow can change significantly during storage or transport at room temperature, making reliable studies of gene expression impossible. PAXgene Bone Marrow RNA Tubes protect RNA molecules from degradation and immediately stabilize the RNA in the sample for storage at room temperature, refrigerated, or frozen.
절차
Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is then transferred into a PAXgene Bone Marrow RNA Tube. The RNA in the sample is immediately stabilized for storage at room temperature, refrigerated, or frozen. The PAXgene Bone Marrow RNA Kit provides an easy and rapid procedure for isolation and purification of intracellular RNA from human whole bone marrow samples. The kit uses silica-membrane technology in a spin-column format for efficient purification of intact, high-quality RNA.
응용 분야
RNA purified using the PAXgene Bone Marrow RNA Kit is ready for use in a wide range of downstream applications, including
- Real-time RT-PCR analysis of specific transcripts
- Array analysis of complex expression profiles
지원되는 데이터 및 수치
Stability of 5 transcripts at room temperature.
The changes in mRNA levels (ΔCT) from day 0 to day 2 for the samples in figure "RNA stability at room temperature" were calculated for each transcript quantified. The minimal changes and small standard deviation for samples stabilized in PAXgene Bone Marrow RNA Tubes contrasts with the significant changes for samples stored in EDTA tubes.
Specifications
| Features | Specifications |
|---|---|
| Tube size | 16 x 125 mm |
| Label type | Paper |
| Draw volume | 2 ml |
| Quantity | 50/box |
| Additive/concentration | Additive 11.1 ml |
| Closure type/color | BD Hemogard/Red |
| Stabilization | up to 3 days at room temperature (15–25°C); up to 5 days at 2–8°C; long-term at –20°C or –70°C |
리소스
Brochures & Guides (3)
Safety Data Sheets (1)
Scientific Posters (2)
Gene Expression Analysis (1)
Kit Handbooks (3)
Certificates of Analysis (1)
FAQ
Where can I find additional information for PreAnalytiX PAXgene products?
How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
What RNA yields are expected with the PAXgene Bone Marrow RNA System?
Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?
What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?