Sequencing grade의 plasmid DNA miniprep을 초고속으로 분리 정제할 수 있습니다
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특징
- One-step lysis — 실험 단계 간소화
- 재현성있는 분리 정제 양 — downstream application을 위한 충분한 plasmid DNA
- 일반적인 molecular biology applications에 적합한 순수한 plasmid
- Ready-to-use plasmid DNA in just 9 minutes
제품 세부 정보
The QuickLyse Miniprep Kit provides a rapid spin-column-based method for isolating sequencing-grade plasmid DNA. Bacterial cell resuspension, lysis, and DNA binding are achieved in a single buffer, enabling fast DNA purification with minimal hands-on time.
성능
The QIAGEN QuickLyse Miniprep Kit uses one-step lysis technology for ultrafast preparation of up to 15 µg plasmid DNA. The quality of the DNA ensures robust performance in applications, such as sequencing, restriction analyses, and cloning (see figure " Reproducible yields").
The QuickLyse Miniprep Kit yields sufficient plasmid DNA for any downstream application, including sequencing (see figure " Long read-lengths in automated sequencing"). Up to 15 µg of high-copy plasmid DNA can be obtained from 1.5 ml of bacterial culture (see figure " High yields of high-quality plasmid DNA"). Isolated plasmid DNA has OD260/280 ratios of 1.7–1.9, indicating that the quality and purity is suitable for all sensitive applications. Sequencing read lengths of over 700 bases are easily achieved.
그림 참조
원리
Unlike most protocols that use a 3-step lysis procedure, the QuickLyse Miniprep Kit combines enzymatic and osmotic processes to lyse bacterial cells in a single, 3-minute step. In addition, QuickLyse technology uses fewer buffers, simplifying handling and saving time: 24 plasmid DNA minipreps can be prepared in less than 22 minutes.
절차
Bacterial cells are resuspended and lysed and DNA is bound in a single buffer, enabling fast DNA purification with minimal hands-on time. After a 3-minute lysis step, the clear lysate is applied directly to a QuickLyse Spin Column. Plasmid DNA binds to the column membrane and is eluted after a single wash step (see flowchart " QuickLyse procedure"). The isolated DNA can be used directly in standard applications, including automated sequencing, PCR, restriction analysis, and cloning.
그림 참조
응용 분야
The QuickLyse Miniprep Kit yields sufficient plasmid DNA for any downstream application, including automated sequencing.
지원되는 데이터 및 수치
High yields of high-quality plasmid DNA.
Typical DNA yield and purity from 1.5 ml of bacterial culture (LB media) for different cells and vectors.
Specifications
| Features | Specifications |
|---|---|
| Applications | Automated sequencing, PCR, restriction analysis, cloning etc. |
| Time per run or prep per run | 9 min |
| Culture volume/starting material | 1.5 ml culture volume |
| Plasmid type | High-copy, cosmid DNA |
| Elution volume | 50 µl |
| Samples per run (throughput) | 1–24 samples per run |
| Technology | QuickLyse technology |
| Yield | up to 15 µg |
| Processing | Manual (centrifugation) |
리소스
Kit Handbooks (2)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?
3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?
Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?