TissueRuptor

Disposable probes를 이용하여 low-throughput으로 샘플을 균일하게 파쇄할 수 있습니다

Products

TissueRuptor은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.

특징

각각의 샘플마다 새로운 probe를 사용함으로써 cross-contamination제거되는 일회용 probe 사용
빠르고 효과적인 파쇄 — 다양한 sample type에서의 안정적인 파쇄
시간 절감 효과 — 각각의 sample을 파쇄한 후, 일회용 probe는 버림
최적화된 protocols — 검증된 QIAGEN 정제기술 통합
Flexibility — 여러가지 샘플 형태, lysis buffer, 파쇄용기를 자유롭게 사용가능

제품 세부 정보

The TissueRuptor is a handheld rotor-stator homogenizer that provides rapid, efficient, and flexible disruption of human, animal, and plant samples for a wide range of downstream applications. The TissueRuptor uses transparent disposable probes, which helps to minimize the risk of cross-contamination and enables visual control of the sample disruption process. Use of a disposable probe also provides time savings as the probe does not have to be cleaned after homogenization of each sample.

성능

QIAGEN has tested use of the TissueRuptor in combination with proven QIAGEN sample preparation kits. Sample disruption with the TissueRuptor ensures maximal yields of nucleic acids and proteins that perform well in downstream applications such as PCR, western blotting, and enzyme assays (see figures "" and ""). For most tissues, disruption and homogenization using the TissueRuptor gives comparable results to traditional rotor–stator homogenization (see figure "Pure RNA with high RIN values"). Effective disruption of tissue samples using the TissueRuptor allows reproducible purification of high-quality DNA and RNA using QIAGEN nucleic acid purification kits (see figures "Pure RNA with high RIN values", "Reproducible purification of high-quality genomic DNA", and "Efficient disruption and homogenization of animal tissues").

그림 참조

Successful purification of phosphoproteins.

High performance results in PCR.

원리

Genotyping, gene expression, and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA, and protein. The TissueRuptor system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table).

The TissueRuptor is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA, and protein. Optimized protocols integrate sample disruption with biomolecule purification, enabling a streamlined, efficient workflow. In addition, a range of automated solutions allow purification and analysis of biomolecules at low to high throughputs.

QIAGEN also provides RNAlater RNA Stabilization Reagent (to stabilize RNA) and Allprotect Tissue Reagent (to stabilize DNA, RNA, and protein). Tissues collected in these reagents can also be easily disrupted with the TissueRuptor.

QIAGEN purification kits compatible with QIAGEN disruption systems
Analyte purified	Sample type	QIAGEN kit
RNA	Easy-to-lyse tissue	RNeasy Kits
		RNeasy Plus Kits
		RNeasy Protect Kits
RNA	Fiber-rich tissue	RNeasy Fibrous Tissue Kits
RNA	All types of tissue	RNeasy Lipid Tissue Kits
RNA	All types of tissue	RNeasy Universal Tissue Kits
RNA	Plant tissue	RNeasy Plant Mini Kit
RNA	Yeast	RNeasy Kits
RNA	Bacteria	RNeasy Protect Bacteria Kits
microRNA	All types of tissue	miRNeasy Kits
DNA	Human tissue	QIAamp DNA Kits
DNA	Animal tissue	DNeasy Blood & Tissue Kits
Protein	Tissue	Qproteome Mammalian Protein Prep Kit
Phosphoprotein	Tissue	PhosphoProtein Purification Kit
Glycoprotein	Tissue	Qproteome Glycoprotein Kits
DNA and RNA	Tissue	AllPrep DNA/RNA Kits
DNA, RNA, and protein	Tissue	AllPrep DNA/RNA/Protein Mini Kit

절차

The TissueRuptor is a portable device with a TissueRuptor Disposable Probe. The blade of the probe rotates at a high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Samples that can be processed include human, animal, and plant tissues (for plant tissues that are more difficult to disrupt, the TissueLyser II is recommended). Disruption at full speed for as little as 30 seconds is usually sufficient to release nucleic acids or proteins from starting material.

After disruption of a sample, the TissueRuptor Disposable Probe can be discarded, and a new probe can be used to disrupt the next sample. Time is saved and cross-contamination is avoided, as there is no need to clean and reuse the same probe. In addition, the TissueRuptor Disposable Probe is transparent, which allows visual monitoring of the sample disruption process.

응용 분야

The TissueRuptor enables efficient disruption and homogenization of human, plant, and animal samples, including clotted blood. Purification of RNA, DNA, total nucleic acids, or protein can then be performed using QIAGEN kits.

지원되는 데이터 및 수치

Reproducible purification of high-quality genomic DNA.

Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.

Efficient disruption and homogenization of animal tissues.

Specifications

Features	Specifications
Disruption principle	Simultaneous disruption and homogenization by rotation of the blade of the disposable probe
Technology	Handheld rotor-stator homogenizer
Kits compatible with instrument	All Kits for purification of RNA/DNA/protein
Technical data	AUS/EU/UK: 230 V, 144 W, 50/60 Hz, 5000-35,000 rpm; NA: 120 V, 144 W, 50/60 Hz, 5000-35,000 rpm; Japan: 100 V, 125 W, 50/60 Hz, 5000-28,000 rpm
Typical run time	15–120 seconds
Features	Rapid, effective disruption of a range of sample types. Disposable probes minimize risk of cross-contamination. Time savings through use of disposable probes. Visual monitoring of disruption using transparent probes. Seamless integration with QIAGEN sample technologies.
Throughput	1 sample per run

리소스

FAQ

Yes, the stainless steel probe can be autoclaved.

FAQ ID -9141

Yield and integrity of genomic DNA depend on the disruption and homogenization method used with the Allprep DNA/RNA Kits.

Homogenization with the TissueRuptor (or other rotor–stator homogenizer, such as the Polytron) or the TissueLyser results in greater DNA fragmentation (depending on homogenization time and intensity). However, shorter DNA fragments are easier to elute, so DNA yields will be higher.

In contast, homogenization using the QIAshredder (or a syringe and needle) is more gentle, resulting in less DNA fragmentation. However, longer DNA fragments are more difficult to elute, so DNA yields may be lower.

FAQ ID -1751

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.

FAQ ID -374

The minimum volume that should be used is 180 µl in a suitably sized vessel (e.g., 2 ml microcentrifuge tube).

FAQ ID -9144

We recommend using either the TissueRuptor or the TissueLyser for homogenization of tissues stored in Allprotect Tissue Reagent.

Since tissues incubated in Allprotect Tissue Reagent are harder than fresh tissues, longer homogenization times might be necessary (for details, see the Allprotect Tissue Reagent Handbook).

Furthermore, we do not recommend using rotor–stator homogenizers with a metal probe (e.g., Polytron) if proteins will be purified subsequently, as the metal probe can heat the sample.

FAQ ID -1572

Buffer ATL can produce foam when used in conjunction with the TissueRuptor. You can add 0.5% final volume of Reagent DX (cat. no. 19088) to prevent the formation of foam.

FAQ ID -9143

We do not recommend to autoclave any part of the TissueRuptor, except the probe adapter for disposable probes.

FAQ ID -9142

Disposable probes can be autoclaved up to 5 times (15 min, 120°C).

FAQ ID -9140

We do not recommend using the probes more than 5 times. Several options for sterilizing and decontaminating the probes can be found in the TissueRuptor User Manual and TissueRuptor Handbook.

FAQ ID -9146

TissueRuptor Disposable Probes (catalog no. 990890) have a diameter of 7 mm (0.3 in) and are 110 mm (4.3 in) long. They are supplied as a pack of 25.

TissueRuptor Steel Probe (catalog no. 9017341) has a diameter of 7 mm (0.3 in) and are 85 mm (3.3 in) long. They are supplied as 1 per pack.

To place an order or to enquire pricing, please contact QIAGEN Customer Care.

FAQ ID -9145