| Cell Line Species/Tissue: |
human
/
acute monocytic leukemia |
| Transfection Reagent: |
Attractene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI (+1mM sodium pyrovate, 9µg/ml human insulin) |
Percent Serum (%):
|
10% |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
8x 10^4 (seeded one day before transfection) |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0,5 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
2,5 µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
48 h |
Transfection Efficiency (%):
|
27 % |
| |
| Any modifications to the protocol?:
|
| Yes, addition of 300 µl medium 3 hours after transfection |
| Notes: |
| |
| References: |
| |