| Cell Line Species/Tissue: |
Human
/
Muscle |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI 1640, 1 mM sodium pyruvate, penicillin (100 IU/ml)/streptomycin( 100 µg/ml) |
Percent Serum (%):
|
10 % foetal calf serum |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
no information |
Number of Cells:
|
|
Percent Confluence(%):
|
no information |
Amount of Nucleic Acid (µg):
|
no information |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
no information |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| The non-classical MHC molecule HLA-G protects human muscle cells from immune-mediated lysis: implications for myoblast transplantation and gene therapy
Heinz Wiendl, Meike Mitsdoerffer, Valeska Hofmeister, Joerg Wischhusen, Elisabeth H. Weiss, Johannes Dichgans, Hanns Lochmuller, Reinhard Hohlfeld, Arthur Melms and Michael Weller
Brain (2003), 126, 176±185 |