Transfection Cell Database - Detailed View


Cell Line: MOLT-4

Cell Line Species/Tissue: Human / lymphoblastic leukemia 
Transfection Reagent: TransMessenger 
Nucleic Acid:
DNA 
Growth Medium:
RPMI 1640 
Percent Serum (%):
10% 
Reporter System:
GFP 
Plasmid Purification Method:
QIAGEN EndoFree Plasmid Kit 
Plate Format:
24 well 
Number of Cells:
200.000 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
1,2µg 
Amount of Enhancer (µl):
4,8µl 
Amount of Reagent (µl):
6µl 
Complex Incubation on Cells (hrs):
48 h 
Analysis Performed Post-Transfection (hrs):
48 h 
Transfection Efficiency (%):
3% 
Knockdown Efficiency (%):  
 
Any modifications to the protocol?:
no complex removal; cells plated in 100 µl RPMI (no serum), complex added in a volume of 100 µl; addition of 200 µl RPMI/ 20% FCS after 3h  
Notes:
 
References: