Transfection Cell Database - Detailed View


Cell Line: Vero

Cell Line Species/Tissue: African green monkey / Kidney 
Transfection Reagent: TransMessenger 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
MEM, Pen Strep GLN 
Percent Serum (%):
10 % 
Reporter System:
GFP 
Plasmid Purification Method:
 
Plate Format:
NA 
Number of Cells:
 
Percent Confluence(%):
80-99% 
Amount of Nucleic Acid (µg):
2 µg 
Amount of Enhancer (µl):
4 µl 
Amount of Reagent (µl):
8 µl 
Complex Incubation on Cells (hrs):
3,5 h 
Analysis Performed Post-Transfection (hrs):
4,8 h 
Transfection Efficiency (%):
65 % 
Knockdown Efficiency (%):  
 
Any modifications to the protocol?:
 
Notes:
Tried Effectene and Electroporationin parallel - did not give as good results  
References: