| Cell Line Species/Tissue: |
mouse
/
kidney tubular epthelial cells |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
MEM |
Percent Serum (%):
|
10 |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
24-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.1 - 0.2 |
Amount of Enhancer (µl):
|
0.8 |
Amount of Reagent (µl):
|
2.5 |
Complex Incubation on Cells (hrs):
|
48 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
35-45% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Transfection efficiency determined by X-gal staining. |
| References: |
| |