QIAamp Virus BioRobot 9604 Kit

For automated purification of viral DNA and RNA from cell-free body fluids on the BioRobot 9604 workstation

Products

The QIAamp Virus BioRobot 9604 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

QIAamp Virus BioRobot 9604 Kit (12)

Cat. No. / ID: 965662

For 12 x 96 nucleic acid preps: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), carrier RNA, caps

¥776,000

Features

Rapid isolation of high-quality, ready-to-use viral DNA and RNA
No organic extraction or alcohol precipitation
Consistent, high yields
Removal of contaminants and inhibitors

Product Details

The QIAamp Virus BioRobot 9604 Kit provides automated viral nucleic acid purification on the BioRobot 9604 using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours for up to 96 samples, including bar code reading and complete process documentation, with no hands-on time during the run.

Performance

QIAamp 96 plates provide well-to-well uniformity in RNA and DNA recovery for reliable results in downstream applications. QIAamp technology yields viral RNA and DNA from cell-free body fluids ready to use in PCR and blotting procedures.

Principle

No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in efficient wash steps, leaving pure nucleic acids to be eluted in a buffer provided with the kit.

Procedure

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use nucleic acid is then eluted in a low-salt buffer using the BioRobot 9604 (see flowchart " Protocol").

Highly pure, viral RNA and DNA can be isolated from 96 cell-free fluid samples in less than 2.5 hours. This kit can be used to isolate nucleic acids from human and animal viruses. QIAamp sample preparation technology is fully licensed.

See figures

QIAamp Virus BioRobot 9604 protocol.

Applications

The QIAamp Virus BioRobot 9604 Kit provides proven QIAamp technology in a convenient 96-well format for automated high-throughput purification of viral nucleic acids on the BioRobot 9604. The kit provides purification of nucleic acids from 200 µl or less of samples, including:

Plasma
Serum
CSF
Other cell-free body fluids
Cell-culture supernatants

Supporting data and figures

QIAamp Virus BioRobot 9604 protocol.

Specifications

Features	Specifications
Applications	PCR, blotting
For automated processing	BioRobot 9604 Workstation
Main sample type	Serum, plasma
Elution volume	50 µl
Format	96-well plates
Processing	Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Viral DNA, viral RNA
Technology	Silica technology
Sample amount	<200 µl
Time per run or per prep	<2.5 hours
Yield	>90% recovery

Resources

For simultaneous purification of viral RNA and DNA from plasma, serum, cell-culture supernatants, and cell-free body fluids using the BioRobot 9604 workstation

Publications

Oseltamivir-resistant influenza A 2009 H1N1 virus in immunocompromised patients.

Couturier BA; Bender JM; Schwarz MA; Pavia AT; Hanson KE; She RC;

Influenza Other Respir Viruses; 2010; 4 (4):199-204 2010 Jul PMID:20836794

First reported outbreak of diarrhea due to adenovirus infection in a hematology unit for adults.

Jalal H; Bibby DF; Tang JW; Bennett J; Kyriakou C; Peggs K; Cubitt D; Brink NS; Ward KN; Tedder RS;

J Clin Microbiol; 2005; 43 (6):2575-80 2005 Jun PMID:15956366

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods.

Yam WC; Chan KH; Chow KH; Poon LL; Lam HY; Yuen KY; Seto WH; Peiris JS;

J Clin Virol; 2005; 33 (1):19-24 2005 May PMID:15797361

A rapid method for simultaneous detection of phenotypic resistance to inhibitors of protease and reverse transcriptase in recombinant human immunodeficiency virus type 1 isolates from patients treated with antiretroviral drugs.

Hertogs K; de Béthune MP; Miller V; Ivens T; Schel P; Van Cauwenberge A; Van Den Eynde C; Van Gerwen V; Azijn H; Van Houtte M; Peeters F; Staszewski S; Conant M; Bloor S; Kemp S; Larder B; Pauwels R;

Antimicrob Agents Chemother; 1998; 42 (2):269-76 1998 Feb PMID:9527771

FAQ

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12