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QIAamp Media MDx Kit (12)
Cat. No. / ID: 965752
For 12 x 96 preps: 12 QIAamp 96 Plates, Buffers, Proteinase K, S-Blocks, Disposable Troughs, Racks with Elution Microtubes CL (0.4 ml), Carrier RNA, Top Elute Fluid, Caps, Tape Pad
The QIAamp Media MDx Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Purification from a variety of liquid transport media
- Fully automated procedure with no centrifugation
- Purification of viral and other DNA
- High-quality DNA with efficient removal of contaminants
Product Details
The QIAamp Media MDx Kit provides a fully automated procedure for purification of DNA from liquid media, such as cervical swab transport media. The fully automated process on the BioRobot MDx workstation, including bar code reading, load check, and complete process documentation, requires only 225 minutes for 96 samples, with no hands-on time.
Performance
Purified nucleic acids are free of proteins, nucleases, and other impurities. Highly reproducible yields enable accurate results in downstream assays (see figure " Highly reproducible yields"). The high-quality nucleic acids are ready to use in downstream assays, such as real-time PCR (see figure " Linear yields of high-quality nucleic acids").
See figures
Principle
The QIAamp Media MDx Kit uses well-established technology for purification of nucleic acids from liquid media. The kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, for fully automated, simultaneous processing of up to ninety-six 265 µl samples on the BioRobot MDx.
Procedure
Liquid transport media can crosslink cells and form cellular aggregates that are difficult to lyse. Optimized buffers in the QIAamp Media MDx Kit efficiently lyse these samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane (see flowchart " Protocol"). Alcohol is added and lysates are loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities, including alcohols and phosphate, and pure, ready-to-use DNA is then eluted in a low-salt buffer. Patent-pending, computer-controlled vacuum technology ensures that volatile contaminants in liquid cytology media, such as alcohols, are efficiently removed.
See figures
Applications
The QIAamp Media MDx Kit can be used for purification of cellular, bacterial, and viral nucleic acids from a variety of sources, including:
- Liquid cytology media with alcohol (e.g., PreservCyt and SurePath)
- Phosphate-buffered liquid transport media (e.g., M4RT)
- Urine
- Dried blood spots, blood cards, and swabs
Supporting data and figures
QIAamp Media MDx protocol.
QIAamp Media MDx protocol.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time PCR |
| Elution volume | 120 µl |
| Main sample type | Liquid media |
| Processing | Automated |
| Technology | Silica technology |
| Time per run or per prep | 200 minutes (96 samples) |
| Sample amount | 265 µl |
| Format | 96-well plate |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral DNA and RNA, bacterial DNA and RNA, cellular DNA and RNA |
| For automated processing | BioRobot MDx Workstation |
| Yield | Varies |
Resources
Kit Handbooks (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?