QIAamp DSP Virus Kit

For purification of viral nucleic acids from human plasma and serum for in vitro diagnostic use

Features

Universal purification system compatible with other IVD products
High-quality viral nucleic acids
Concentration with 20 µl or 60 µl eluates
Sample volumes of 500 µl
Rapid purification and minimal risk of cross-contamination

Product Details

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA for in vitro diagnostic use.

Performance

Viral nucleic acids purified using the QIAamp DSP Virus Kit are ready to use in sensitive downstream applications, such as those based on enzymatic amplification, including PCR and RT-PCR.

Principle

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA. The QIAamp silica-based membrane binds nucleic acids in the lysed sample, while the rest of the lysate is rapidly removed by vacuum pressure. The bound nucleic acids are efficiently washed to remove contaminants and then eluted in a volume of 20 µl or 60 µl.

Procedure

The QIAamp DSP Virus Kit Procedure includes the following steps: lyse, bind, wash (3x), dry spin, and elute. Viral nucleic acids are purified from plasma or serum using a vacuum manifold, such as the QIAvac 24 Plus (see flowchart, "").

See figures

QIAamp DSP Virus Kit procedure.

Applications

Viral nucleic acids can be purified from plasma or serum samples. Samples can contain the anticoagulants citrate or EDTA, and can be either fresh, lyophilized, or frozen (provided they were not thawed and refrozen).

The QIAamp DSP Virus Kit provides purification of nucleic acids from a wide range of viruses. The kit is compatible with a wide range of upstream sample collection systems and downstream applications, and therefore can be easily integrated into diagnostic workflows.

Supporting data and figures

QIAamp DSP Virus Kit procedure.

Universal nucleic acid purification system that easily fits into diagnostic workflows.

Specifications

Features	Specifications
Applications	Downstream detection procedures in molecular diagnostics, such as PCR
Elution volume	20 µl, 60 µl
Main sample type	Serum, plasma
CE/FDA/IVD compatible	CE/IVD
Format	Sample tubes
Processing	Manual (vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Viral DNA, viral RNA
Sample amount	500 µl
Technology	Silica technology
Yield	Varies

Resources

Publications

Occurrence of hepatitis A virus genotype III in Germany requires the adaptation of commercially available diagnostic test systems.

Heitmann A; Laue T; Schottstedt V; Dotzauer A; Pichl L;

Transfusion; 2005; 45 (7):1097-105 2005 Jul PMID:15987353

FAQ

It is possible to use a general laboratory vacuum system with the QIAamp DSP Virus Kit. However, this will require validation in-house. The CE-marked QIAamp Kits have been developed, field-tested and validated with the QIAvac 24 Plus vacuum system (QIAvac 24 Plus, QIAvac Connecting System and Vacuum Pump). Using this system with the QIAamp DSP Virus Kit will avoid lengthy validation procedures for the enduser.

FAQ ID -789

When processing samples with the QIAamp DSP DNA Blood Mini Kit and the QIAamp DSP Virus Kit, the vacuum pressure should be below -800 mbar. The QIAvac 24 Plus and the QIAvac Connecting System should be tested according to Appendix B of the QIAvac 24 Plus Handbook before performing a nucleic acid purification procedure.

If you would like to use an equivalent general laboratory vacuum system, make sure that the minimum vacuum pressure is reached. Performance of the QIAamp DSP DNA Blood Mini or Virus Kits in combination with a house-vacuum system has to be validated by the researcher.

FAQ ID -1033

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

FAQ ID -528

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12