Buffer G2

Buffer G2 is a component of QIAGEN Blood & Cell Culture DNA Kits, but is also provided with several other kits (e.g., for automation on the EZ1 Advanced instrument). Usually Buffer G2 is used in combination with Proteinase K or QIAGEN Protease for efficient lysis.

Buffer G2 (General Lysis Buffer ) consists of: 800 mM guanidine hydrochloride; 30 mM Tris•Cl, pH 8.0; 30 mM EDTA, pH 8.0; 5% Tween 20; 0.5% Triton X-100.

How to prepare Buffer G2: Dissolve 76.42 g guanidine hydrochloride, 11.17g Na2EDTA•2H2O, and 3.63 g Tris base in 600 ml distilled water. Add 250 ml 20% Tween 20 solution and 50 ml 10% Triton X-100 solution. Adjust the pH to 8.0 with NaOH. Adjust the volume to 1 liter with distilled water.

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.