QIAsymphony RGQ

遺伝子解析のニーズに応える精製、アッセイセットアップ、検出までの包括的な自動化

Products

The QIAsymphony SP is intended to be used only in combination with QIAsymphony Kits indicated for use with the QIAsymphony SP for the applications described in the kit handbooks. If used in combination with QIAGEN kits indicated for use with the QIAsymphony AS instrument, the QIAsymphony AS is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAsymphony AS instrument is used with kits other than QIAGEN kits, it is the user's responsibility to validate the performance of such product combination for any particular application. The Rotor-Gene Q, if used in combination with QIAGEN kits indicated for use with the Rotor-Gene Q instrument, is intended for the applications described in the respective QIAGEN kits handbooks. If the Rotor-Gene Q instrument is used with kits other than QIAGEN kits, it is the user's responsibility to validate the performance of such product combination for any particular application.
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QIAsymphony RGQ, System

Cat. No. / ID:   9001850

QIAsymphony SP, QIAsymphony AS, Rotor-Gene Q 5plex HRM; includes required accessories and consumables, installation, and training; includes 1-year warranty on parts and labor
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QIAsymphony Cabinet SP/AS

Cat. No. / ID:   9020246

Accessory for correct positioning of the QIAsymphony SP/AS instruments

特徴

  • 多くの解析に適応可能
  • ワークフローの最適化および実用化
  • 使いやすさと簡便性を最大化
  • 法規制に沿ったアッセイや独自の検査に対応
  • 多彩なアプリケーションに対応可能なデザイン

製品詳細

QIAGENの新しいワークフロー・ソリューションは、QIAsymphony SPによるサンプル調製からQIAsymphony ASによるアッセイセットアップ、そしてリアルタイムPCR装置Rotor-Gene Qで多彩なアッセイに対応しています。QIAGENはさらにQIAsymphonyシリーズを拡充するように開発を続けています。

パフォーマンス

QIAsymphony RGQとartus QS-RGQ Kitsの組み合わせにより感度と特異性の高い検出を以下のサンプルで行なえます:

図参照

原理

QIAsymphony RGQ はサンプル調製から結果獲得までの全操作の自動化を実現し、1 回のランで最大96 サンプルを処理するため、時間を短縮できます。各種専用キットは遺伝子解析のオープンシステムにもクローズドシステムにも対応します。

QIAsymphony RGQは、QIAsymphony SP/AS 装置とRotor-Gene Q の組み合わせにより全ワークフローを統合したシステムの自動化を実現します。QIAsymphony SP は様々な種類のサンプルからのサンプル調製を自動化します。QIAsymphony AS は再現性と信頼性の高いPCR アッセイのセットアップを実現し、複雑なPCR を使用した遺伝子解析を日常的に行なうラボに最適です。QIAsymphony AS は1 ランあるいは1 サンプルあたり複数のアッセイをセットアップでき、CE-IVDに完全に対応するワークフローのための artus QS-RGQ 製品を用いた実験や独自のアッセイにも適しています。Rotor-Gene QはCE-IVD-marked artus QS-RGQ kitsあるいは独自のアッセイと組み合わせて感度と精度の高いリアルタイムPCR解析を実現します。

QIAsymphony RGQでCE-IVDに完全に対応するワークフロー用キット
キット 装置 メソッド
QIAsymphony DSP Virus/Pathogen Kits QIAsymphony SP 各種サンプルタイプからウイルス核酸あるいはバクテリアDNAの精製
artus QS-RGQ Kits QIAsymphony AS リアルタイムPCR(マルチプレックス;サンプルとインターナルコントロール)

QIAsymphony RGQでの独自のテスト用キット
アプリケーション キット 装置 メソッド
精製 QIAsymphony DSP Virus/Pathogen Kits QIAsymphony SP 各種サンプルタイプからウイルス核酸あるいはバクテリアDNAの精製
QIAsymphony DSP AXpH DNA Kit QIAsymphony SP 液状細胞培地からのDNA精製
QIAsymphony Certal Kits QIAsymphony SP バイオプロセスにおける精製バッファー、培養細胞上清サンプル、ワクチンプレップから残留しているホスト細胞DNAやウイルス核酸の精製
アッセイセットアップ — 病原体検出 QuantiFast Pathogen +IC Kits QIAsymphony AS 配列特異的プローブを用いたマルチプレックス・リアルタイムPCR/RT-PCR
QuantiTect Virus Kits QIAsymphony AS 同一チューブ中の複数のウイルスDNAおよびRNAターゲットの高感度検出
QIAGEN OneStep RT-PCR Kit QIAsymphony AS 簡単なセットアップでどのようなRNAテンプレートでも高感度RT-PCRを実現
アッセイセットアップ — 遺伝子発現 Rotor-Gene SYBR® Green Kits QIAsymphony AS SYBR® Green I を用いた超高速で高精度なリアルタイムPCR/RT-PCR
Rotor-Gene Probe Kits QIAsymphony AS 配列特異的プローブを用いた超高速で高精度なリアルタイムPCR/RT-PCR
Rotor-Gene Multiplex Kits QIAsymphony AS 配列特異的プローブを用いた超高速で高精度なマルチプレックス・リアルタイムPCR/RT-PCR
アッセイセットアップ — 残存DNAの検出 Certal Detection Kits QIAsymphony AS resDNAと一般的な内部コントロールのduplexリアルタイムPCR検出

操作手順

QIAsymphony RGQ ワークフローではまずQIAsymphony SP で核酸精製を開始します。サンプル調製操作で精製した核酸を含む溶出液をQIAsymphony ASモジュールにトランスファーし、アッセイのセットアップを行ないます。その後アッセイをRotor-Gene Q にトランスファーし、PCR とデータ解析を行ないます。

それぞれの実験ワークフロー条件にマッチするように、システムオペレーションには2 つのモード“integrated”と“independent”があります。“Integrated”モードではQIAsymphony SP で調製したサンプルがQIAsymphony AS に直接自動移動するため、マニュアル操作ステップは少なくなります。また、QIAsymphony SP とAS は“Independent”モードで個々に操作可能なため高い適応性を持ちます。(参照“ QIAsymphony ワークフロー”)。

図参照

アプリケーション

QIAsymphony RGQ は遺伝子解析のニーズに対応可能な、非常に多様性のあるシステムです。QIAsymphony RGQ は市販のアッセイやラボごとの独自の試験を一台のプラットホームで実施できます。

オープンシステムおよびクローズドシステム用の多様なアプリケーションには以下のようなものが含まれます:

  • artus移植/免疫抑制パネル(CMV、EBV、HSV、BK virus、VZV)
  • artus 血液媒介ウイルスパネル(HIV, HCV, and HBV)
  • 独自の試験ワークフロー

裏付けデータと数値

Specifications

FeaturesSpecifications
Elution volumeQIAsymphony SP — 30–500 µl (application dependent)
ProcessingQIAsymphony SP/AS — automated
Storage temperatureQIAsymphony SP/AS — 5–40°C (41ºF to 104ºF) in manufacturer's package Rotor-Gene Q — 15 to 30ºC (59ºF to 86ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 1K2 (IEC 60721-3-1)
FeaturesRotor-Gene Q — dynamic range, 10 orders of magnitude
Operating temperatureQIAsymphony SP/AS — 15–32°C (59–89.6ºF) Rotor-Gene Q — 18–30ºC (64–86ºF)
HumidityQIAsymphony SP/AS — relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C Rotor-Gene Q — relative humidity of 10–75% (noncondensing)
Pollution levelQIAsymphony SP/AS — 2 Rotor-Gene Q — 2; Environmental class 3K2 (IEC 60721-3-3)
Overvoltage categoryII
Samples per run (throughput)QIAsymphony SP — 1–96 samples in batches of 24 Rotor-Gene Q — tubes 0.2 ml; strip tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; up to 100 samples per run using a Rotor-Disc 100
Heat dissipation/thermal loadRotor-Gene Q — average, 0.183 kW (632 BTU/hour); peak, 0.458 kW (1578 BTU/hour)
Protocols/main application on this instrumentQIAsymphony SP — purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials QIAsymphony AS — PCR setup Rotor-Gene Q — gene expression analysis, microRNA detection, virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM)
TechnologyQIAsymphony SP — QIAGEN magnetic-particles chemistry Rotor-Gene Q — real-time PCR cycler
Input volumeQIAsymphony SP — minimum input volume: 200 µl (application dependent)
Optical SystemRotor-Gene Q — up to 6 channels spanning UV to infra-red wavelengths; excitation sources: high energy light-emitting diodes; detector: photomultiplier; acquisition time: 4 s; the software allows to create new excitation/detection wavelength combinations
Kits or Application Packs designed for this instrumentQIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits Rotor-Gene Q — artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit
Place of operationFor indoor use only
PowerQIAsymphony SP/AS — 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages Rotor-Gene Q — 100–240 V AC, 50–60 Hz, 520 VA (peak); Power consumption 8 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5A 250 V fuse
ソフトウェア Rotor-Gene Q — Rotor-Gene Q software, supplied on the provided installation CD
Thermal performanceRotor-Gene Q — temperature range, 35 to 99ºC; temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); temperature resolution, ±0.02ºC (smallest programmable increment); temperature uniformity, ±0.02ºC; ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling
Typical run timeRotor-Gene Q — 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent)
重量QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg; Rotor-Gene Q, 12.5 kg (27.6 lb.) (standard configuration)
Transportation conditionsQIAsymphony SP/AS — –25 to 70°C (–13ºF to 158ºF) in manufacturer's package Rotor-Gene Q — –25 to 60ºC (–13ºF to 140ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 2K2 (IEC 60721-3-2)
WarrantyRotor-Gene Q — 1 year on instrument; lifetime guarantee on excitation LEDs
Instrument dimensionsQIAsymphony SP/AS — QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm Rotor-Gene Q — width, 37 cm (14.6 in.); height, 28.6 cm (11.3 in.); depth (without cables), 42 cm (16.5 in.); depth (door open), 53.8 cm (21.2 in.)
AltitudeUp to 2000 m (6500 ft)

リソース

アプリケーション/プロトコール (29)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
Supporting Software and Tools (11)
For QIAsymphony software version 5.0.4
QIAsymphony Management Console operating software for QIAsymphony software version 4.1
QIAsymphony Management Console operating software for QIAsymphony software version 4.0.3
Russian language package for QIAsymphony software version 5.0.4
Language Package – Spanish
SOFTWARE (781KB)
Spanish language package for QIAsymphony software version 5.0.4
Language Package – French
SOFTWARE (882KB)
French language package for QIAsymphony software version 5.0.4
Language Package – Chinese
SOFTWARE (747KB)
Chinese language package for QIAsymphony software version 5.0.4
Norwegian language package for QIAsymphony software version 5.0.4
Language Package – Italian
SOFTWARE (643KB)
Italian language package for QIAsymphony software version 5.0.4
Language Package – German
SOFTWARE (830KB)
German language package for QIAsymphony software version 5.0.4
プロトコール (6)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
機器ユーザーマニュアル (14)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with the QIAsymphony SP/AS instruments (software version 4.0)
For use with the QIAsymphony SP/AS instruments (software version 4.0)
For use with the QIAsymphony SP/AS instruments (software version 4.0)
For use with the QIAsymphony SP/AS instruments (software version 4.0)
Guideline for ISO 20836:2020
For Rotor-Gene Q instruments using Rotor-Gene Q Software version 2.3.1
For use with Rotor-Gene Q Software version 2.3.4
For use with QIAsymphony SP/AS instruments (software versions 4.0 and 4.1)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
機器テクニカル資料 (16)
QIAsymphony Cabinet SP/AS documents
Script change document for software version 4.0
Differences between QIAsymphony software versions
For use with QIAsymphony SP/AS instruments (software version 5.0)
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
Script change document for software version 4.0
Script change document for software version 4.0
Script change document for software version 4.0
Script change document for software version 4.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony Cabinet SP/AS document
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
Software document for QIAsymphony software version 4.0.1
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
Labware Files and Documents (3)
パンフレット (4)
For automated purification of nucleic acids or 6xHis-tagged recombinant proteins from a wide variety of sample types
Success Story
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

The QIAsymphony RNA kit uses RLT plus as lysis buffer. Can samples lysed in RLT and then stored in the freezer be used on the QIAsymphony?

The RLT buffer is more viscous than the RLT plus buffer. Extraction will work fine, but there may be some magnetic bead carryover to the eluate.

FAQ ID - 3533
There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do?

The barcode on the adapter at the cooling elution position is read by default. This can be changed in the configuration settings which are available from the tools menu if you have logged in as Supervisor. The option is on the tab Process SP1 and is called ”On which elution slots should the bar codes be scanned”. To disable the barcode reading, put the setting to ”none”.

FAQ ID - 3532
What is the elution volume for all CE-marked artus QS-Rotor-Gene Q assays?
The elution volume for all artus QS-RGQ assays is 60µl.
FAQ ID -2432
What is the batch size of the QIASymphony Rotor-Gene Q and how many PCR´s can be processed per assay module run?

With the QS-RGQ the user can process 1 to 4 batches with 1 to 24 reactions per specific batch. RNA assays are limited to 72 reactions in total.

For PCR:  216 reactions for DNA and 72 reactions for RNA viruses can be performed.

FAQ ID -2433
Is barcode reading supported by QS-RGQ?
Yes. The procedure in more detail is described in the instrument handbook.
FAQ ID -2437
Is it possible to process samples on the AS-module while SP is running?
Yes. A connected as well as an independent operation between QS-SP and QS-AS is possible.
FAQ ID -2435