BioSprint DNA Blood Kits

For rapid and economical DNA purification from a wide range of sample types

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BioSprint 96 DNA Blood Kit (48)

Cat. No. / ID: 940054

For 48 preps: Large 96-Rod Covers, 96-Well Microplates MP, S-Blocks, MagAttract Suspension G, buffers and reagents

For instrument

Biosprint 96

Biosprint 15

Preparations

384

BioSprint DNA Blood Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

High-throughput isolation of DNA
Maximal flexibility to accommodate challenging sample material
Complete removal of contamination and inhibitors
No organic extraction or alcohol precipitation

Product Details

BioSprint DNA Blood Kits provide all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots and buccal swabs using proven magnetic-particle technology on the BioSprint 15 or BioSprint 96.

Performance

DNA can be successfully purified from a variety of sample types (see table “Typical yields from a range of sample types”).

Typical yields from a range of sample types

Sample type	Amount of sample	DNA (µg)
		BioSprint 15	BioSprint 96
Muscle, bovine	25 mg	16.2 ± 2.5	13.5 ± 1.5
Heart, bovine	25 mg	5.9 ± 2.6	3.4 ± 0.6
Spleen, bovine	25 mg	69.1 ± 23.6	59.1 ± 4.8
Lung, bovine	25 mg	13.8 ± 7.2	14.7 ± 5.5
Liver, bovine	25 mg	77.8 ± 29.4	74.0 ± 22.3
Kidney, bovine	25 mg	26.2 ± 18.8	33.5 ± 5.4
Ear, sheep	30 mg	20.31 ± 1.8	–
Tail, mouse	1.2 cm (~25 mg)	32.7 ± 4.6	30.9 ± 4.5
HL-60 cultured cells	2 x 10⁶ cells	10.1 ± 4.7	9.6 ± 5.6
Blood, human (5–7 x 10⁶ cells per ml)	200 µl	4.5–9.0	4.5–9.0
Blood, bovine	200 µl	6.2–8.0	4.4–7.0
Blood, sheep	200 µl	5.6–11.2	1.2–8.0
Blood, pig	200 µl	4.5–9.0	4.5–9.0
Blood, dog	200 µl	6.7–12.3	6.9–16.1
Blood, cat	100 µl	4.4–8.3	2.9–6.3
Blood, mouse	100 µl	2.0–8.0	1.5–3.0
Blood, rat	100 µl	1.0–4.0	1.0–3.0
Blood, bird	10 µl*	15.0 ±6.3	–
Blood, fish	5 µl*	7.1–10.0	–
Dried blood spots, 903 Specimen Collection Paper	1 punch (6 mm [1/4 inch] diameter)	0.30	0.10
Dried blood spots, FTA Card	1 punch (6 mm [1/4 inch] diameter)	0.12	0.05
Buccal swabs	1 swab	0.8–2.0	0.8–2.0

Genomic DNA was purified from the indicated samples. DNA was eluted in 200 µl Buffer AE.

* Sample diluted to 200 µl.

Purified DNA is up to 50 kb in size (see figures " Purification of high-quality genomic DNA from mouse tail" and " Reproducible purification of DNA from sheep ear tissue"). The DNA is ready to use in all downstream applications, including Southern blotting and enzyme applications such as PCR (see figure " High PCR performance of purified DNA").

See figures

Purification of high-quality genomic DNA from mouse tail.

Reproducible purification of DNA from sheep ear tissue.

High PCR performance of purified DNA.

Principle

BioSprint DNA Blood Kits are used with the BioSprint 15 or BioSprint 96. The kits use magnetic-particle technology for rapid DNA purification from cells, tissue and blood or blood-related products. BioSprint instruments control an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube or well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure " Efficient processing of magnetic particles"). Since the instruments transfer magnetic particles instead of liquids, they use minimal amounts of reagents, enabling cost-efficient sample preparation.

See figures

Efficient processing of magnetic particles.

Procedure

Genomic DNA can be purified from cells, tissue and blood. Lysed samples are loaded into the instrument, which then performs all steps until pure DNA is obtained (see figure " BioSprint DNA procedure"). The kits provide all the necessary reagents and plasticware for sample preparation, and are available in different sizes, allowing cost-efficient use of consumables.

For purification of genomic DNA from tissue, dried blood spots or buccal swabs, Buffer ATL and QIAGEN Proteinase K are required (not supplied with BioSprint DNA Blood Kits).

See figures

BioSprint DNA procedure.

Applications

BioSprint DNA Blood Kits purify DNA for use in a range of downstream applications, including:

Southern blotting
PCR and real-time PCR
Genotyping

Comparison of BioSprint DNA Blood Kits

Features	BioSprint 15 DNA Blood Kit	BioSprint 96 DNA Blood Kit
Applications	PCR, real-time PCR, genotyping, blotting	PCR, real-time PCR, genotyping, blotting
Elution volume	200 µl	Custom
For automated processing	BioSprint 15 workstation	BioSprint 96 workstation
Format	5-tube strip, S-Block	S-Block, 96-well plate
Main sample type	Blood, tissue, cells	Blood, tissue, cells
Processing	Automated	Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	DNA	DNA
Sample amount	100 µl/25 mg/2 x 10e6	100–250 µl
Technology	Magnetic particles	Magnetic particles
Time per run or per prep	45 minutes	45 minutes
Yield	Varies	Varies

Supporting data and figures

Purification of high-quality genomic DNA from mouse tail.

Mouse tail samples were treated with 180 µl Buffer ATL and 20 µl QIAGEN Proteinase K at 56°C overnight. Genomic DNA was purified from the lysed tissue samples using the BioSprint 96 DNA Blood Kit with the BioSprint 96 DNA Tissue protocol. DNA was eluted in 200 µl elution buffer. Eluates (2 µl) from 8 out of 96 samples were visualized by agarose gel electrophoresis. M: markers.

Resources

For purification of DNA from human whole blood, animal whole blood, buffy coat, cultured cells, tissues, rodent tails, buccal swabs, and dried blood spots using the BioSprint 15 workstation

For purification of DNA from human whole blood, animal whole blood, buffy coat, cultured cells, tissues, rodent tails, buccal swabs, and dried blood spots using the BioSprint 96 workstation

FAQ

No, the BioSprint Instruments should not be operated in a cool room. Optimal operation temperature for BioSprint is 10-40°C. Operation at 4°C may harm the instruments.

FAQ ID -938

Yes, please refer to the protocols mentioned in the following handbooks:

BioSprint 15 DNA Handbook

BioSprint 96 DNA Handbook

FAQ ID -983

Yes. We have shown that DNA purified with the BioSprint DNA Kits (e.g., the BioSprint 15 DNA Blood Kit, BioSprint 15 DNA Plant Kit, BioSprint 96 DNA Blood Kit and BioSprint 96 DNA Plant Kit) is stable for at least 2 years when being stored at either 2-8°C or -20°C. Our data indicate however that DNA stability is dependent upon storage conditions. For additional information see also FAQ 518.

FAQ ID -1318

Yes, please follow either of the Supplementary Protocols:

FAQ ID -977

Add 1/10 volume of 3 M Na-Acetate pH 5.2, and 2 to 2.5 volumes of ice-cold 100% ethanol to the DNA sample
Mix, and store at –20°C for at least 1 h to precipitate the DNA
Recover the precipitated DNA by centrifugation at full speed in a microcentrifuge for 15–20 min
Pour off the ethanol and wash the pellet twice with room-temperature 70% ethanol
Allow the DNA pellet to air-dry
resuspend the DNA in a suitable volume of sterile TE buffer or distilled water

FAQ ID -305

Yes, please follow either of the Supplementary Protocols:

FAQ ID -982

Yes, please follow either of the Supplementary Protocols:

FAQ ID -979

The BioSprint 96 instrument has a magnetic-particle collecting efficiency above 95%. However, to avoid particle carryover from the eluate plate to subsequent applications, you can use a plate magnet while pipetting.

FAQ ID -2073

High-quality genomic DNA can be purified from up to 96 pre-lysed samples in just 20 minutes. Including setup, the total time per run for the blood and buffy coat protocol is 45 minutes. For the other protocols (e.g. tissue), the run time will be slightly higher depending on the time of pre-treatment.

FAQ ID -622

Measure the absorbance of the eluate obtained on the BioSprint 96 instruments at 260 nm, 280 nm and 320 nm. Carryover of magnetic particles in the eluates may affect the A260 and A280 readings, but should not affect the performance of the DNA in downstream applications. Subtract the absorbance reading obtained at 320 nm from the readings obtained at 260 nm and 280 nm to correct for the presence of residual magnetic particles.

FAQ ID -773

Bisulfite conversion of unmethylated cytosines into uracils with the EpiTect Bisulfite Kit works on DNA irrespective of the source organism. The DNA template needs to be of high purity for efficient conversion. We recommend to use genomic DNA extracted with our DNA isolation kits for clinical or animal and plant samples as a template for the EpiTect Bisulfite Kit.

FAQ ID -1209

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

Yes, the BioSprint 15/96 instruments are stand-alone instruments and do not need to be connected to a computer for the actual run. However, a computer is needed to import and export protocols.

FAQ ID -620