QIAsymphony DSP DNA Kits

1~96 サンプルからのDNA 自動精製

Products

QIAsymphony DSP DNA Kits are intended for in-vitro diagnostic use.
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QIAsymphony DSP DNA Mini Kit (192)

Cat. No. / ID:   937236

For 192 preps of 200 µl each: Includes 2 reagent cartridges and enzyme racks and accessories.
QIAsymphony DSP DNA Mini Kit (192) は体外診断用です。
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QIAsymphony DSP DNA Midi Kit (96)

Cat. No. / ID:   937255

For 96 preps of 1000 µl each or 144 preps of 400 µl each: Includes 2 reagent cartridges and enzyme racks and accessories.

特徴

  • 多様なサンプルタイプからのDNA精製
  • 使いやすい斬新な充填済み試薬カートリッジ
  • 標準化された調製法で高い再現性
  • バーコードによりサンプルや試薬の使用履歴トラッキングが可能

製品詳細

QIAsymphony DSP DNA KitとQIAsymphony SPの組み合わせにより、ヒト全血、バフィコート、組織、FFPE組織、細胞培養液、バクテリア培養液からDNAを、またヒト全血からのウイルスDNAを自動精製し、遺伝子解析に利用できます。キットは200 μlから1,000 μl までのサンプル量に応じて、それぞれMini、Midi のフォーマットがあります。組織、FFPE組織、細胞培養液、バクテリア培養液のプロトコールに関してはマニュアルで前処理が必要です。至適化済みのプロトコールにより高純度DNA が高収量で得られ、溶出容量が調節可能なのでダウンストリームごとにDNA濃度の至適化が簡単にできます。斬新で充填済みの試薬カートリッジにより、セットアップ時間を短縮しマニュアルでの取り扱いは最小限に抑えられます。バーコードによる読み取りにより、試薬のトラッキングを完全に行なえます。

原理

QIAsymphony DSP DNA Kitは、ヒト全血、バフィコート、組織、FFPE組織、細胞培養液、バクテリア培養液からDNAを、またヒト全血からウイルスDNAを精製し、遺伝子解析に利用できます。

QIAsymphony DSP DNA Kitは、シリカゲルをベースにした迅速で効率的なDNA精製法と磁性粒子の簡便な取り扱いを組み合わせています(図" QIAsymphony SP原理の模式図”)。革新的で即使用可能な試薬カートリッジは、精製工程に必要なすべての試薬(酵素も含む)が充填済みです。 QIAsymphony SPはカートリッジを自動的に開けて、実験環境からのコンタミのリスクを回避します(図" 充填済みのプレパックカートリッジ”)。ワークテーブルのセットアップが簡単かつ迅速に行なえ、貴重な時間を節約できます。QIAsymphony SP のボックスに最大2種類の試薬カートリッジを入れるだけで準備完了です。同じキットの試薬カートリッジではなく、別々のキットの試薬カートリッジをセットすれば、一回のランで96サンプルから2種類の精製工程が可能です。選択したサンプル数だけの試薬量が使用されるため、コストを完璧にコントロールできます。

図参照

操作手順

精製操作は感染性の高いサンプルを安全かつ再現性よく取り扱えるようにデザインされ、溶解、結合、洗浄、溶出の4ステップで構成されています(図"QIAsymphony DSP DNA操作手順”)。至適化済みのプロトコールにより、タンパク質、ヌクレアーゼ、その他の不純物を効率的に除去し、高品質核酸を精製します。QIAsymphony DSP DNA Kitで精製した核酸は増幅や酵素反応などダウンストリームアプリケーションですぐに使用できます。ユーザーは適切な溶出量を選択できます。
図参照

アプリケーション

QIAsymphony DSP DNA Kitは、シークエンシングや リアルタイム定量 PCRあるいはRT-PCRを用いた高感度検出を含むダウンストリームアプリケーションで即使用可能な高品質核酸を精製します。

裏付けデータと数値

リソース

アプリケーション/プロトコール (56)
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
Application/Protocol Documents for IVD Use (21)
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
DNA_Blood_200_V7_DSP protocol

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

DNA_Blood_400_V6_DSP protocol

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

DNA_Blood_1000_V7_DSP protocol

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

DNA_Buffy_Coat_200_V7 DSP protocol

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

DNA_Buffy_Coat_400_V6 DSP protocol

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

VirusBlood200_V5_DSP protocol

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP protocols

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
MSDS (1)
Download Safety Data Sheets for QIAGEN product components.
パフォーマンスデータ (2)
QIAsymphony DSP DNA Kit Performance Characteristics_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
実験器具関連資料 (2)
QIAsymphony DSP DNA Kit Labware List_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
For use with software version 5.0 or higher
Technical Information and Important Notes (1)
Labware Files and Documents (1)
For use with the QIAsymphony SP/AS instruments (software version 4.0)
Application/Protocol Documents for User-Validated Use (2)
Automated DNA purification from cultured cells and bacterial cultures using the QIAsymphony DSP DNA Mini Kit in combination with the QIAsymphony SP.

The QIAsymphony DSP DNA Mini Kit in combination with the Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP (user-validated) protocols for purification of total DNA from cultured cells and bacterial cultures is intended for molecular biology applications. This combination is not intended for the diagnosis, prevention, or treatment of a disease.

For use with software version 4.0
Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP; Version 2
キットハンドブック (2)
QIAsymphony DSP DNA Kit Handbook_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Safety Data Sheets (1)
Certificates of Analysis (1)
Performance Data (2)
QIAsymphony DSP DNA Kit Performance Characteristics_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Application/Protocol Documents (56)
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
For use with software version 4.0
Kit Handbooks (2)
QIAsymphony DSP DNA Kit Handbook_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Labware Documents (2)
QIAsymphony DSP DNA Kit Labware List_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
For use with software version 5.0 or higher

FAQ

What sample types can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit?
Tissue and FFPE tissue can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP.
FAQ ID -2925
How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?

Tissue: If no information about the expected yield is available, we recommend starting with 25 mg sample material. Depending on the yield obtained, the sample size can be increased in subsequent preparations to a maximum of 50 mg.

FFPE: Up to 4 sections, each with a thickness of up to 10 µm, or 8 sections with a thickness of up to 5 µm and a surface area of up to 250 mm2, can be combined in one preparation.

FAQ ID -2927
Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
Yes. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using the QIAsymphony DSP DNA Mini Kit. Tissue samples are lysed by proteinase K digestion. FFPE tissue samples are deparaffinized with Deparaffinization Solution or xylene/ethanol and lysed by proteinase K digestion. Insoluble material is pelleted by centrifugation. Lysates are placed on the QIAsymphony SP for purification of DNA (see protocol sheets for details).
FAQ ID -2926
What sample volumes can be used with the tissue HC and LC protocols on the QIAsymphony SP?
After pretreatment, 220 µl of the lysates must be transferred to the QIAsymphony SP to ensure that 200 µl of sample is processed.
FAQ ID -2992
What are the recommended lysis times for tissue samples on the QIAsymphony SP?
Lysis time varies depending on the tissue type processed. For recommended lysis times refer to the tissue protocol sheets. If lysis is incomplete after recommended lysis time, as indicated by the presence of insoluble material or highly viscous lysates, lysis time can be prolonged or insoluble material can be removed by centrifugation. Overnight lysis is possible and does not affect the preparation.
FAQ ID -2993
What sample types should be processed with the low content (LC) and high content (HC) protocols for DNA extraction on the QIAsymphony SP?
Tissues will give increased DNA yields when processed with the high content protocol, but the low content protocol in combination with a small elution volume (50µl) may be used if high DNA concentration is required. For FFPE tissue we recommend using the low content protocol.
FAQ ID -2989
Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
Yes. The DNA tissue protocols on the QIAsymphony SP purify genomic and mitochondrial DNA.
FAQ ID -2991