| Cell Line Species/Tissue: |
Monkey
/
|
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
100-mm dish |
Number of Cells:
|
2 million |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
10µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
80µl |
Complex Incubation on Cells (hrs):
|
48h |
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
95% |
| |
| Any modifications to the protocol?:
|
| none |
| Notes: |
| Used FuGene before and achieved 90% transfection efficiency. |
| References: |
| |