| Cell Line Species/Tissue: |
adult male Sprague-Dawley rats
/
|
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
Eagle’s modified essential medium, 5 units/ml penicillin, and 5 µg/ml streptomycin |
Percent Serum (%):
|
10% fetral bovine |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
glass-bottomed confocal chambers (~4.9 cm2) |
Number of Cells:
|
no information |
Percent Confluence(%):
|
no information |
Amount of Nucleic Acid (µg):
|
0.5-1 µg |
Amount of Enhancer (µl):
|
no information |
Amount of Reagent (µl):
|
no information |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| Ventricular choline transport. A role for organic cation transporter 2 expressed in choroid plexus
Sweet, D.H., Miller, D.S. and Pritchard, J.B.
J. Biol. Chem. (2001) 276(45), 41611-41619 |