| Cell Line Species/Tissue: |
Rat
/
heart muscle |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 |
Reporter System:
|
Luciferase |
Plasmid Purification Method:
|
|
Plate Format:
|
60-mm dish |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
3 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
|
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Co-transfection resulted in a 6-fold increase in reporter activity over basal level |
| References: |
| Characterization of the TBX5 binding site and analysis of mutations that cause Holt-Oram syndrome, Tushar K. Ghosh et al.; Human Molecular Genetics, 2001, Vol.10, No.18, 1983-1994 |