| Cell Line Species/Tissue: |
Opossum
/
Kidney proximal tubulus |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM/F12 (1:1) with Glutamax-I (Gibco); 20 mM HEPES pH 7.4; 50 IU/ml penicillin; 50 ug/ml streptomycin |
Percent Serum (%):
|
10 FCS |
Reporter System:
|
|
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
12-well plate |
Number of Cells:
|
456000 |
Percent Confluence(%):
|
60 |
Amount of Nucleic Acid (µg):
|
0.3 |
Amount of Enhancer (µl):
|
2.4 |
Amount of Reagent (µl):
|
18 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
120 |
Transfection Efficiency (%):
|
90% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |