| Cell Line Species/Tissue: |
rat
/
adrenal pheochromocytoma |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
10% FBS + 5% HS |
Percent Serum (%):
|
10% FBS + 5% HS |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1.0 |
Amount of Enhancer (µl):
|
8.0 |
Amount of Reagent (µl):
|
4.0 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
25% |
| |
| Any modifications to the protocol?:
|
| more DNA and enhancer (best results with titration) |
| Notes: |
| |
| References: |
| |