| Cell Line Species/Tissue: |
rabbit
/
aortic smooth muscle |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI + Pen/Strep |
Percent Serum (%):
|
10 |
Reporter System:
|
GFP, pEGFP-N1 (ClonTech) |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.4 |
Amount of Enhancer (µl):
|
3.2 |
Amount of Reagent (µl):
|
10 |
Complex Incubation on Cells (hrs):
|
6 |
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
40-50% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| DNA amount optimized from 0.1 to 2 ug. |
| References: |
| |