| Cell Line Species/Tissue: |
human
/
kidney |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
MEM |
Percent Serum (%):
|
10% |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
|
Plate Format:
|
Eppendorf tube |
Number of Cells:
|
|
Percent Confluence(%):
|
60-80% |
Amount of Nucleic Acid (µg):
|
4 µg |
Amount of Enhancer (µl):
|
32 µl |
Amount of Reagent (µl):
|
100 µl |
Complex Incubation on Cells (hrs):
|
24 h |
Analysis Performed Post-Transfection (hrs):
|
26 h |
Transfection Efficiency (%):
|
30-40% |
| |
| Any modifications to the protocol?:
|
| cotransfection with 3 plasmids |
| Notes: |
| |
| References: |
| |