Transfection Cell Database - Detailed View


Cell Line: Primary HAEC

Cell Line Species/Tissue: Human / Aortic Endothelial Cells 
Transfection Reagent: RNAiFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
EGM-2 
Percent Serum (%):
2% 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
24-well 
Number of Cells:
60.000 
Percent Confluence(%):
70% 
Amount of Nucleic Acid (µg):
0.5 µg (~100 nM) 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
3 µl 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
48 h 
Transfection Efficiency (%):
>90% 
Knockdown Efficiency (%): >90%  
 
Any modifications to the protocol?:
complex formation works with Buffer EC-R or medium  
Notes:
 
References: