| Cell Line Species/Tissue: |
Rat
/
hepatoma |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM with antibiotics |
Percent Serum (%):
|
10% |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
~45 |
Amount of Nucleic Acid (µg):
|
2 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
8 |
Complex Incubation on Cells (hrs):
|
2 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
Beta-gal activity 1.588 |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Beta-galactosidase activity was measured as conversion of CPRG to 564 nm absorbing product after 30 minute incubation |
| References: |
| |