| Cell Line Species/Tissue: |
rat
/
adrenal pheochromocytoma |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM + 50ng/m/NGF |
Percent Serum (%):
|
5-10 |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kits |
Plate Format:
|
60-mm dish |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
5 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
25 |
Complex Incubation on Cells (hrs):
|
2-3 |
Analysis Performed Post-Transfection (hrs):
|
up to 72 |
Transfection Efficiency (%):
|
25-30% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Differentiated cells were transfected in 0.05% FBS (low-serum), yielding 5% efficiency (compared to 25-30% for undifferentiated PC-12 cells.) |
| References: |
| Kelly-Spratt, K. (1998) Transfection of PC-12 cells: a model system for primary neuronal cells. QIAGEN News 1998 No. 4, 3 |