| Cell Line Species/Tissue: |
human
/
osteosarcoma bone, epithelial |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DME |
Percent Serum (%):
|
10 |
Reporter System:
|
|
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
12.5 x 10e3/cm2 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.2 |
Amount of Enhancer (µl):
|
1.6 |
Amount of Reagent (µl):
|
2 |
Complex Incubation on Cells (hrs):
|
30 |
Analysis Performed Post-Transfection (hrs):
|
30 |
Transfection Efficiency (%):
|
500,000 RLU |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| DNA:Effectene ratios tested 1:10 to 1:60. 1:10 worked best. |
| References: |
| |