My samples are expected to have low concentrations. Can I load more sample into the workflow or more lysate into the PCR reaction?

The amount of sample tested for the sample preparation is 250 µL. This is the best condition for both samples containing a high or low amount of background cells. Depending on your sample, you can optimize the amount of sample to isolate. You can load a maximum of 22.6 µL eluate per RT-dPCR reaction. Due to the high sensitivity of the workflow, this should be enough to get a signal for very low concentrations of mycoplasma contamination as described in the different Pharmacopoeias.