What cell line does QIAGEN use for quality control testing of the SureSilencing shRNA Plasmids?
We use HEK-239H (human embryonic kidney) cells for our quality control assays. The key to successful RNA interference is the ability to transfect cells. There are no reported cell line or even species-specific design criteria for shRNA. Once the cell is transfected with an effective sequence designed by an experimentally validated algorithm, the shRNA will knock down the expression of the gene of interest