Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?

 Yes, we have a Supplementary Protocol for 60 mm dishes and 96-well plates:

• 'Transient Transfecton of HeLa-S3 cells in 60 mm dishes' (TFP01)
• 'Transient Transfecton of HeLa-S3 cells in 96-well plates' (TFP02)