How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase?
Use of a PCR enhancer containing Betaine, DTT, BSA, and DMSO (3) may also be used to improve yield of complex targets (especially targets ≥ 3 kb). Increasing the extension time, the template concentration, or polymerase concentration to 2 U/50 µL may also be helpful.